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Journal of Biomedical Optics • Open Access

Structured light scatteroscopy

Paper Abstract

A new imaging approach, structured light scatteroscopy (SLS), is demonstrated, which offers rapid wide-field imaging of microscopic morphological variations in bulk tissue surfaces. Elastic scattering of light offers exquisite sensitivity to ultrastructural changes at multiple size scales ranging from nanometers to millimeters, but in bulk tissues the confounding effects of molecular absorption and strong multiple scattering of light often lead to a dramatic reduction in scatter contrast and specificity. It is demonstrated that the SLS using structured high spatial frequency illumination and detection to probe the tissue achieves direct, absorption-independent, high-resolution maps of the scattering response. The scattering response is observed to be dependent on both the wavelength and spatial frequency of choice, indicating a potential for multiscale probing of ultrastructural changes in superficial tissue layers. This methodology can be easily applied in most wide-field imaging systems.

Paper Details

Date Published: 24 July 2014
PDF: 3 pages
J. Biomed. Opt. 19(7) 070504 doi: 10.1117/1.JBO.19.7.070504
Published in: Journal of Biomedical Optics Volume 19, Issue 7
Show Author Affiliations
Venkataramanan Krishnaswamy, Thayer School of Engineering at Dartmouth (United States)
Jonathan T. Elliott, Thayer School of Engineering at Dartmouth (United States)
David M. McClatchy, Thayer School of Engineering at Dartmouth (United States)
Richard J. Barth, Geisel School of Medicine (United States)
Wendy A. Wells, Geisel School of Medicine (United States)
Brian W. Pogue, Thayer School of Engineering at Dartmouth (United States)
Keith D. Paulsen, Thayer School of Engineering at Dartmouth (United States)


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