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Journal of Biomedical Optics • Open Access

Deep two-photon microscopic imaging through brain tissue using the second singlet state from fluorescent agent chlorophyll α in spinach leaf
Author(s): Lingyan Shi; Adrián Rodríguez-Contreras; Yury Budansky; Yang Pu; Thien An Nguyen; Robert R. Alfano

Paper Abstract

Two-photon (2P) excitation of the second singlet (S2) state was studied to achieve deep optical microscopic imaging in brain tissue when both the excitation (800 nm) and emission (685 nm) wavelengths lie in the “tissue optical window” (650 to 950 nm). S2 state technique was used to investigate chlorophyll α (Chl α) fluorescence inside a spinach leaf under a thick layer of freshly sliced rat brain tissue in combination with 2P microscopic imaging. Strong emission at the peak wavelength of 685 nm under the 2P S2 state of Chl α enabled the imaging depth up to 450 μm through rat brain tissue.

Paper Details

Date Published: 26 June 2014
PDF: 5 pages
J. Biomed. Opt. 19(6) 066009 doi: 10.1117/1.JBO.19.6.066009
Published in: Journal of Biomedical Optics Volume 19, Issue 6
Show Author Affiliations
Lingyan Shi, The City College of New York (United States)
Adrián Rodríguez-Contreras, The City Univ. of New York (United States)
Yury Budansky, The City College of New York (United States)
Yang Pu, The City College of New York (United States)
Thien An Nguyen, The City Univ. of New York (United States)
Robert R. Alfano, The City College of New York (United States)


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