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Journal of Biomedical Optics

Rotational multispectral fluorescence lifetime imaging and intravascular ultrasound: bimodal system for intravascular applications
Author(s): Dinglong M. Ma; Julien Bec; Diego R. Yankelevich; Dimitris S. Gorpas; Hussain Fatakdawala; Laura Marcu
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Paper Abstract

We report the development and validation of a hybrid intravascular diagnostic system combining multispectral fluorescence lifetime imaging (FLIm) and intravascular ultrasound (IVUS) for cardiovascular imaging applications. A prototype FLIm system based on fluorescence pulse sampling technique providing information on artery biochemical composition was integrated with a commercial IVUS system providing information on artery morphology. A customized 3-Fr bimodal catheter combining a rotational side-view fiberoptic and a 40-MHz IVUS transducer was constructed for sequential helical scanning (rotation and pullback) of tubular structures. Validation of this bimodal approach was conducted in pig heart coronary arteries. Spatial resolution, fluorescence detection efficiency, pulse broadening effect, and lifetime measurement variability of the FLIm system were systematically evaluated. Current results show that this system is capable of temporarily resolving the fluorescence emission simultaneously in multiple spectral channels in a single pullback sequence. Accurate measurements of fluorescence decay characteristics from arterial segments can be obtained rapidly (e.g., 20 mm in 5 s), and accurate co-registration of fluorescence and ultrasound features can be achieved. The current finding demonstrates the compatibility of FLIm instrumentation with <italic<in vivo</italic< clinical investigations and its potential to complement conventional IVUS during catheterization procedures.

Paper Details

Date Published: 4 June 2014
PDF: 11 pages
J. Biomed. Opt. 19(6) 066004 doi: 10.1117/1.JBO.19.6.066004
Published in: Journal of Biomedical Optics Volume 19, Issue 6
Show Author Affiliations
Dinglong M. Ma, Univ. of California, Davis (United States)
Julien Bec, Univ. of California, Davis (United States)
Diego R. Yankelevich, Univ. of California, Davis (United States)
Dimitris S. Gorpas, Univ. of California, Davis (United States)
Hussain Fatakdawala, Univ. of California, Davis (United States)
Laura Marcu, Univ. of California, Davis (United States)

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