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Journal of Biomedical Optics • Open Access

Two-step phase-shifting fluorescence incoherent holographic microscopy
Author(s): Wan Qin; Xiaoqi Yang; Yingying Li; Xiang Peng; Hai Yao; Xinghua Qu; Bruce Z. Gao

Paper Abstract

Fluorescence holographic microscope (FINCHSCOPE) is a motionless fluorescence holographic imaging technique based on Fresnel incoherent correlation holography (FINCH) that shows promise in reconstructing three-dimensional fluorescence images of biological specimens with three holograms. We report a developing two-step phase-shifting method that reduces the required number of holograms from three to two. Using this method, we resolved microscopic fluorescent beads that were three-dimensionally distributed at different depths with two interferograms captured by a CCD camera. The method enables the FINCHSCOPE to work in conjunction with the frame-straddling technique and significantly enhance imaging speed.

Paper Details

Date Published: 27 June 2014
PDF: 3 pages
J. Biomed. Opt. 19(6) 060503 doi: 10.1117/1.JBO.19.6.060503
Published in: Journal of Biomedical Optics Volume 19, Issue 6
Show Author Affiliations
Wan Qin, Clemson Univ. (United States)
Xiaoqi Yang, Clemson Univ. (United States)
Yingying Li, Shenzhen Univ. (China)
Xiang Peng, Shenzhen Univ. (China)
Hai Yao, Clemson Univ. (United States)
Xinghua Qu, Tianjin Univ. (China)
Bruce Z. Gao, Clemson Univ. (United States)

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