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Journal of Biomedical Optics • Open Access

Simultaneous imaging of cellular morphology and multiple biomarkers using an acousto-optic tunable filter–based bright field microscope
Author(s): Elliot S. Wachman; Stanley J. Geyer; Joel M. Recht; Jon D. Ward; Bill G. Zhang; Murray K. Reed; Christopher N. Pannell

Paper Abstract

An acousto-optic tunable filter (AOTF)-based multispectral imaging microscope system allows the combination of cellular morphology and multiple biomarker stainings on a single microscope slide. We describe advances in AOTF technology that have greatly improved spectral purity, field uniformity, and image quality. A multispectral imaging bright field microscope using these advances demonstrates pathology results that have great potential for clinical use.

Paper Details

Date Published: 9 May 2014
PDF: 14 pages
J. Biomed. Opt. 19(5) 056006 doi: 10.1117/1.JBO.19.5.056006
Published in: Journal of Biomedical Optics Volume 19, Issue 5
Show Author Affiliations
Elliot S. Wachman, Gooch & Housego, New Jersey (United States)
Stanley J. Geyer, Geyer Pathology Services (United States)
Joel M. Recht, Gooch & Housego, New Jersey (United States)
Jon D. Ward, Gooch & Housego plc (United Kingdom)
Bill G. Zhang, Gooch & Housego, Orlando (United States)
Murray K. Reed, Gooch & Housego, Palo Alto (United States)
Christopher N. Pannell, Gooch & Housego, Orlando (United States)


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