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Journal of Biomedical Optics • Open Access

Noninvasive optical cytochrome c oxidase redox state measurements using diffuse optical spectroscopy
Author(s): Jangwoen Lee; Jae Gwan Kim; Sari B. Mahon; David Mukai; David Yoon; Gerry R. Boss; Steven E. Patterson; Gary Rockwood; Gary Isom; Matthew Brenner

Paper Abstract

A major need exists for methods to assess organ oxidative metabolic states in vivo. By contrasting the responses to cyanide (CN) poisoning versus hemorrhage in animal models, we demonstrate that diffuse optical spectroscopy (DOS) can detect cytochrome c oxidase (CcO) redox states. Intermittent decreases in inspired O 2 from 100% to 21% were applied before, during, and after CN poisoning, hemorrhage, and resuscitation in rabbits. Continuous DOS measurements of total hemoglobin, oxyhemoglobin, deoxyhemoglobin, and oxidized and reduced CcO from muscle were obtained. Rabbit hemorrhage was accomplished with stepwise removal of blood, followed by blood resuscitation. CN treated rabbits received 0.166  mg/min NaCN infusion. During hemorrhage, CcO redox state became reduced concurrently with decreases in oxyhemoglobin, resulting from reduced tissue oxygen delivery and hypoxia. In contrast, during CN infusion, CcO redox state decreased while oxyhemoglobin concentration increased due to CN binding and reduction of CcO with resultant inhibition of the electron transport chain. Spectral absorption similarities between hemoglobin and CcO make noninvasive spectroscopic distinction of CcO redox states difficult. By contrasting physiological perturbations of CN poisoning versus hemorrhage, we demonstrate that DOS measured CcO redox state changes are decoupled from hemoglobin concentration measurement changes.

Paper Details

Date Published: 1 May 2014
PDF: 10 pages
J. Biomed. Opt. 19(5) 055001 doi: 10.1117/1.JBO.19.5.055001
Published in: Journal of Biomedical Optics Volume 19, Issue 5
Show Author Affiliations
Jangwoen Lee, Beckman Laser Institute and Medical Clinic (United States)
Jae Gwan Kim, Beckman Laser Institute and Medical Clinic (United States)
Gwangju Institute of Science and Technology (Korea, Republic of)
Sari B. Mahon, Beckman Laser Institute and Medical Clinic (United States)
David Mukai, Beckman Laser Institute and Medical Clinic (United States)
David Yoon, Beckman Laser Institute and Medical Clinic (United States)
Gerry R. Boss, Univ. of California, San Diego (United States)
Steven E. Patterson, Univ. of Minnesota, Twin Cities (United States)
Gary Rockwood, U.S. Army Medical Research Institute of Chemical Defense (United States)
Gary Isom, Purdue Univ. (United States)
Matthew Brenner, Univ. of California, Irvine (United States)
Univ. of California Irvine Medical Ctr. (United States)

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