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Journal of Biomedical Optics

Simple method of DNA stretching on glass substrate for fluorescence imaging and spectroscopy
Author(s): Guru Prakash Neupane; Krishna P. Dhakal; Min Su Kim; Hyunsoo Lee; Martin Guthold; Vincent S. Joseph; Jong-Dal Hong; Jeongyong Kim
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Paper Abstract

We demonstrate a simple method of stretching DNA to its full length, suitable for optical imaging and atomic force microscopy (AFM). Two competing forces on the DNA molecules, which are the electrostatic attraction between positively charged dye molecules (YOYO-1) intercalated into DNA and the negatively charged surface of glass substrate, and the centrifugal force of the rotating substrate, are mainly responsible for the effective stretching and the dispersion of single strands of DNA. The density of stretched DNA molecules could be controlled by the concentration of the dye-stained DNA solution. Stretching of single DNA molecules was confirmed by AFM imaging and the photoluminescence spectra of single DNA molecule stained with YOYO-1 were obtained, suggesting that our method is useful for spectroscopic analysis of DNA at the single molecule level.

Paper Details

Date Published: 9 January 2014
PDF: 7 pages
J. Biomed. Opt. 19(5) 051210 doi: 10.1117/1.JBO.19.5.051210
Published in: Journal of Biomedical Optics Volume 19, Issue 5
Show Author Affiliations
Guru Prakash Neupane, Univ. of Incheon (Korea, Republic of)
Krishna P. Dhakal, Sungkyunkwan Univ. (Korea, Republic of)
Min Su Kim, Institute for Basic Science (Korea, Republic of)
Hyunsoo Lee, Wake Forest Univ. (United States)
Martin Guthold, Wake Forest Univ. (United States)
Vincent S. Joseph, Incheon National Univ. (Korea, Republic of)
Jong-Dal Hong, Incheon National Univ. (Korea, Republic of)
Jeongyong Kim, Sungkyunkwan Univ. (Korea, Republic of)

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