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Journal of Biomedical Optics • Open Access

In vivo imaging of human adipose-derived stem cells in Alzheimer’s disease animal model
Author(s): Sungji Ha; Sangzin Ahn; Saeromi Kim; Yuyoung Joo; Young Hae Chong; Yoo-Hun Suh; Keun-A Chang

Paper Abstract

Stem cell therapy is a promising tool for the treatment of diverse conditions, including neurodegenerative diseases such as Alzheimer’s disease (AD). To understand transplanted stem cell biology, in vivo imaging is necessary. Nanomaterial has great potential for in vivo imaging and several noninvasive methods are used, such as magnetic resonance imaging, positron emission tomography, fluorescence imaging (FI) and near-infrared FI. However, each method has limitations for in vivo imaging. To overcome these limitations, multimodal nanoprobes have been developed. In the present study, we intravenously injected human adipose-derived stem cells (hASCs) that were labeled with a multimodal nanoparticle, LEO-LIVE™-Magnoxide 675 or 797 (BITERIALS, Seoul, Korea), into Tg2576 mice, an AD mouse model. After sequential in vivo tracking using Maestro Imaging System, we found fluorescence signals up to 10 days after injection. We also found strong signals in the brains extracted from hASC-transplanted Tg2576 mice up to 12 days after injection. With these results, we suggest that in vivo imaging with this multimodal nanoparticle may provide a useful tool for stem cell tracking and understanding stem cell biology in other neurodegenerative diseases.

Paper Details

Date Published: 2 December 2013
PDF: 5 pages
J. Biomed. Opt. 19(5) 051206 doi: 10.1117/1.JBO.19.5.051206
Published in: Journal of Biomedical Optics Volume 19, Issue 5
Show Author Affiliations
Sungji Ha, Gachon Univ. of Medicine and Science (Korea, Republic of)
Sangzin Ahn, Seoul National Univ. College of Medicine (Korea, Republic of)
Saeromi Kim, Seoul National Univ. College of Medicine (Korea, Republic of)
Yuyoung Joo, Gachon Univ. of Medicine and Science (Korea, Republic of)
Young Hae Chong, Ewha Womans Univ. (Korea, Republic of)
Yoo-Hun Suh, Seoul National Univ. College of Medicine (Korea, Republic of)
Keun-A Chang, Gachon Univ. of Medicine and Science (Korea, Republic of)


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