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Journal of Biomedical Optics • Open Access

Aptamer-based single-molecule imaging of insulin receptors in living cells
Author(s): Minhyeok Chang; Mijin Kwon; Sooran Kim; Na-Oh Yunn; Daehyung Kim; Sung Ho Ryu; Jong-Bong Lee

Paper Abstract

We present a single-molecule imaging platform that quantitatively explores the spatiotemporal dynamics of individual insulin receptors in living cells. Modified DNA aptamers that specifically recognize insulin receptors (IRs) with a high affinity were selected through the SELEX process. Using quantum dot-labeled aptamers, we successfully imaged and analyzed the diffusive motions of individual IRs in the plasma membranes of a variety of cell lines (HIR, HEK293, HepG2). We further explored the cholesterol-dependent movement of IRs to address whether cholesterol depletion interferes with IRs and found that cholesterol depletion of the plasma membrane by methyl-β -cyclodextrin reduces the mobility of IRs. The aptamer-based single-molecule imaging of IRs will provide better understanding of insulin signal transduction through the dynamics study of IRs in the plasma membrane.

Paper Details

Date Published: 2 December 2013
PDF: 7 pages
J. Biomed. Opt. 19(5) 051204 doi: 10.1117/1.JBO.19.5.051204
Published in: Journal of Biomedical Optics Volume 19, Issue 5
Show Author Affiliations
Minhyeok Chang, Pohang Univ. of Science and Technology (Korea, Republic of)
Mijin Kwon, Ulsan National Institute of Science and Technology (Korea, Republic of)
Sooran Kim, Pohang Univ. of Science and Technology (Korea, Republic of)
Na-Oh Yunn, Pohang Univ. of Science and Technology (Korea, Republic of)
Daehyung Kim, Pohang Univ. of Science and Technology (Korea, Republic of)
Sung Ho Ryu, Pohang Univ. of Science and Technology (Korea, Republic of)
Jong-Bong Lee, Pohang Univ. of Science and Technology (Korea, Republic of)


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