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Journal of Biomedical Optics

Localized surface plasmon resonance based nanobiosensor for biomarker detection of invasive cancer cells
Author(s): Yoochan Hong; Minhee Ku; Eugene Lee; Jin-Suck Suh; Yong-Min Huh; Dae-Sung Yoon; Jaemoon Yang
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Paper Abstract

In this study, we describe the development of a cancer biomarker-sensitive nanobiosensor based on localized surface plasmon resonance that enables recognition for proteolytic activity of membrane type 1 matrix metalloproteinase (MT1-MMP) anchored on invasive cancer cells. First of all, we prepared biomarker-detectable substrate based on gold nanorods (GNRs) using nanoparticle adsorption method. The sensitivity of the sensing chip was confirmed using various solvents that have different refractive indexes. Subsequently, MT1-MMP–specific cleavable peptide was conjugated onto the surface of GNRs, and molecular sensing about proteolytic activity was conducted using MT1-MMP and cell lysates. Collectively, we developed a biomarker detectable sensor, which allows for the effective detection of proteolytic activity about MT1-MMP extracted from invasive cancer cells.

Paper Details

Date Published: 2 December 2013
PDF: 7 pages
J. Biomed. Opt. 19(5) 051202 doi: 10.1117/1.JBO.19.5.051202
Published in: Journal of Biomedical Optics Volume 19, Issue 5
Show Author Affiliations
Yoochan Hong, Yonsei Univ. (Korea, Republic of)
Minhee Ku, Yonsei Univ. College of Medicine (Korea, Republic of)
Eugene Lee, Yonsei Univ. College of Medicine (Korea, Republic of)
Jin-Suck Suh, Yonsei Univ. College of Medicine (Korea, Republic of)
Yong-Min Huh, Yonsei Univ. College of Medicine (Korea, Republic of)
Dae-Sung Yoon, Yonsei Univ. (Korea, Republic of)
Jaemoon Yang, Yonsei Univ. College of Medicine (Korea, Republic of)

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