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Journal of Biomedical Optics

Laser phototherapy triggers the production of reactive oxygen species in oral epithelial cells without inducing DNA damage
Author(s): Caroline Siviero Dillenburg; Luciana Oliveira Almeida; Manoela Domingues Martins; Cristiane Helena Squarize; Rogerio Moraes Castilho
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Paper Abstract

Laser phototherapy (LPT) is widely used in clinical practice to accelerate healing. Although the use of LPT has advantages, the molecular mechanisms involved in the process of accelerated healing and the safety concerns associated with LPT are still poorly understood. We investigated the physiological effects of LPT irradiation on the production and accumulation of reactive oxygen species (ROS), genomic instability, and deoxyribose nucleic acid (DNA) damage in human epithelial cells. In contrast to a high energy density (20  J/cm 2 ), laser administered at a low energy density (4  J/cm 2 ) resulted in the accumulation of ROS. Interestingly, 4  J/cm 2 of LPT did not induce DNA damage, genomic instability, or nuclear influx of the BRCA1 DNA damage repair protein, a known genome protective molecule that actively participates in DNA repair. Our results suggest that administration of low energy densities of LPT induces the accumulation of safe levels of ROS, which may explain the accelerated healing results observed in patients. These findings indicate that epithelial cells have an endowed molecular circuitry that responds to LPT by physiologically inducing accumulation of ROS, which triggers accelerated healing. Importantly, our results suggest that low energy densities of LPT can serve as a safe therapy to accelerate epithelial healing.

Paper Details

Date Published: 29 April 2014
PDF: 8 pages
J. Biomed. Opt. 19(4) 048002 doi: 10.1117/1.JBO.19.4.048002
Published in: Journal of Biomedical Optics Volume 19, Issue 4
Show Author Affiliations
Caroline Siviero Dillenburg, Univ. Federal do Rio Grande do Sul (Brazil)
Luciana Oliveira Almeida, Univ. of Michigan (United States)
Manoela Domingues Martins, Univ. Federal do Rio Grande do Sul (Brazil)
Cristiane Helena Squarize, Univ. of Michigan (United States)
Rogerio Moraes Castilho, Univ. of Michigan (United States)


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