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Journal of Biomedical Optics • Open Access

Accurate single-shot quantitative phase imaging of biological specimens with telecentric digital holographic microscopy
Author(s): Ana I. Doblas; Emilio Sánchez-Ortiga; Manuel Martínez-Corral; Genaro Saavedra; Jorge Garcia-Sucerquia

Paper Abstract

The advantages of using a telecentric imaging system in digital holographic microscopy (DHM) to study biological specimens are highlighted. To this end, the performances of nontelecentric DHM and telecentric DHM are evaluated from the quantitative phase imaging (QPI) point of view. The evaluated stability of the microscope allows single-shot QPI in DHM by using telecentric imaging systems. Quantitative phase maps of a section of the head of the drosophila melanogaster fly and of red blood cells are obtained via single-shot DHM with no numerical postprocessing. With these maps we show that the use of telecentric DHM provides larger field of view for a given magnification and permits more accurate QPI measurements with less number of computational operations.

Paper Details

Date Published: 29 April 2014
PDF: 8 pages
J. Biomed. Opt. 19(4) 046022 doi: 10.1117/1.JBO.19.4.046022
Published in: Journal of Biomedical Optics Volume 19, Issue 4
Show Author Affiliations
Ana I. Doblas, Univ. de València (Spain)
Emilio Sánchez-Ortiga, Univ. de València (Spain)
Manuel Martínez-Corral, Univ. de València (Spain)
Genaro Saavedra, Univ. de València (Spain)
Jorge Garcia-Sucerquia, Univ. de València (Spain)


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