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Journal of Biomedical Optics

Tomographic phase microscopy of living three-dimensional cell cultures
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Paper Abstract

A successful application of self-interference digital holographic microscopy in combination with a sample-rotation-based tomography module for three-dimensional (3-D) label-free quantitative live cell imaging with subcellular resolution is demonstrated. By means of implementation of a hollow optical fiber as the sample cuvette, the observation of living cells in different 3-D matrices is enabled. The fiber delivers a stable and accurate rotation of a cell or cell cluster, providing quantitative phase data for tomographic reconstruction of the 3-D refractive index distribution with an isotropic spatial resolution. We demonstrate that it is possible to clearly distinguish and quantitatively analyze several cells grouped in a “3-D cluster” as well as subcellular organelles like the nucleoli and local internal refractive index changes.

Paper Details

Date Published: 10 April 2014
PDF: 8 pages
J. Biomed. Opt. 19(4) 046009 doi: 10.1117/1.JBO.19.4.046009
Published in: Journal of Biomedical Optics Volume 19, Issue 4
Show Author Affiliations
Arkadiusz Kuś, Warsaw Univ. of Technology (Poland)
Michał Dudek, Warsaw Univ. of Technology (Poland)
Björn Kemper, Univ. Münster (Germany)
Małgorzata Kujawińska, Warsaw Univ. of Technology (Poland)
Angelika Vollmer, Univ. Münster (Germany)

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