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Journal of Biomedical Optics • Open Access

Label-free cell-based assay with spectral-domain optical coherence phase microscopy
Author(s): Suho Ryu; Kyung-A Hyun; Jung Heo; Hyo-Il Jung; Chulmin Joo

Paper Abstract

Quantitative measurement of dynamic responses of unstained living cells is of great importance in many applications ranging from investigation of fundamental cellular functions to drug discoveries. Conventional optical methods for label-free cell-based assay examine cellular structural changes proximal to sensor surfaces under external stimuli, but require dedicated nanostructure-patterned substrates for operation. Here, we present a quantitative imaging method, spectral-domain optical coherence phase microscopy (SD-OCPM), as a viable optical platform for label-free cell-based assay. The instrument is based on a low-coherence interferometric microscope that enables quantitative depth-resolved phase measurement of a transparent specimen with high phase stability. We demonstrate SD-OCPM measurement of dynamic responses of human breast cancer cells (MCF-7) to 2-picolinic acid (PA) and histamine.

Paper Details

Date Published: 7 April 2014
PDF: 7 pages
J. Biomed. Opt. 19(4) 046003 doi: 10.1117/1.JBO.19.4.046003
Published in: Journal of Biomedical Optics Volume 19, Issue 4
Show Author Affiliations
Suho Ryu, Yonsei Univ. (Korea, Republic of)
Kyung-A Hyun, Yonsei Univ. (Korea, Republic of)
Jung Heo, Yonsei Univ. (Korea, Republic of)
Hyo-Il Jung, Yonsei Univ. (Korea, Republic of)
Chulmin Joo, Yonsei Univ. (Korea, Republic of)

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