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Journal of Biomedical Optics

Four-dimensional motility tracking of biological cells by digital holographic microscopy
Author(s): Xiao Yu; Jisoo Hong; Changgeng Liu; Michael Cross; Donald T. Haynie; Myung K. Kim
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Paper Abstract

Three-dimensional profiling and tracking by digital holography microscopy (DHM) provide label-free and quantitative analysis of the characteristics and dynamic processes of objects, since DHM can record real-time data for microscale objects and produce a single hologram containing all the information about their three-dimensional structures. Here, we have utilized DHM to visualize suspended microspheres and microfibers in three dimensions, and record the four-dimensional trajectories of free-swimming cells in the absence of mechanical focus adjustment. The displacement of microfibers due to interactions with cells in three spatial dimensions has been measured as a function of time at subsecond and micrometer levels in a direct and straightforward manner. It has thus been shown that DHM is a highly efficient and versatile means for quantitative tracking and analysis of cell motility.

Paper Details

Date Published: 3 April 2014
PDF: 10 pages
J. Biomed. Opt. 19(4) 045001 doi: 10.1117/1.JBO.19.4.045001
Published in: Journal of Biomedical Optics Volume 19, Issue 4
Show Author Affiliations
Xiao Yu, Univ. of South Florida (United States)
Jisoo Hong, Univ. of South Florida (United States)
Changgeng Liu, Univ. of South Florida (United States)
Michael Cross, Univ. of South Florida (United States)
Donald T. Haynie, Univ. of South Florida (United States)
Myung K. Kim, Univ. of South Florida (United States)

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