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Journal of Biomedical Optics • Open Access

Hemoglobin parameters from diffuse reflectance data
Author(s): Judith R. Mourant; Oana C. Marina; Tiffany M. Hebert; Gurpreet Kaur; Harriet O. Smith

Paper Abstract

Tissue vasculature is altered when cancer develops. Consequently, noninvasive methods of monitoring blood vessel size, density, and oxygenation would be valuable. Simple spectroscopy employing fiber optic probes to measure backscattering can potentially determine hemoglobin parameters. However, heterogeneity of blood distribution, the dependence of the tissue-volume-sampled on scattering and absorption, and the potential compression of tissue all hinder the accurate determination of hemoglobin parameters. We address each of these issues. A simple derivation of a correction factor for the absorption coefficient, μ a , is presented. This correction factor depends not only on the vessel size, as others have shown, but also on the density of blood vessels. Monte Carlo simulations were used to determine the dependence of an effective pathlength of light through tissue which is parameterized as a ninth-order polynomial function of μ a . The hemoglobin bands of backscattering spectra of cervical tissue are fit using these expressions to obtain effective blood vessel size and density, tissue hemoglobin concentration, and oxygenation. Hemoglobin concentration and vessel density were found to depend on the pressure applied during in vivo acquisition of the spectra. It is also shown that determined vessel size depends on the blood hemoglobin concentration used.

Paper Details

Date Published: 26 March 2014
PDF: 10 pages
J. Biomed. Opt. 19(3) 037004 doi: 10.1117/1.JBO.19.3.037004
Published in: Journal of Biomedical Optics Volume 19, Issue 3
Show Author Affiliations
Judith R. Mourant, Los Alamos National Lab. (United States)
Oana C. Marina, Los Alamos National Lab. (United States)
Tiffany M. Hebert, Jack D. Weiler Hospital (United States)
Gurpreet Kaur, Einstein Cancer Ctr. (United States)
Harriet O. Smith, Einstein Cancer Ctr. (United States)

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