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Journal of Biomedical Optics

Steady-state and time-resolved fluorescence spectroscopic characterization of urine of healthy subjects and cervical cancer patients
Author(s): Ramu Rajasekaran; Prakasa Rao Aruna; Dornadula Koteeswaran; Ganesan Bharanidharan; Munusamy Baludavid; Singaravelu Ganesan
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Paper Abstract

Steady-state and time-resolved fluorescence spectroscopy were employed in the discrimination of cervical cancer patients from healthy subjects using urine samples. Fluorescence emission at 390 and 440 nm was considered to monitor the fluorescence of indoxyl sulfate and neopterin. Significant spectral differences were observed between healthy and cancer subjects. Different ratio parameters were calculated from the spectral intensity at 280- and 350-nm excitation and were subjected to stepwise linear discriminant analysis. In total, 84.0% of samples were correctly classified at 280 nm and 96.4% were correctly classified at 350 nm. The fluorescence decay kinetics of urine samples at 390-nm emission was best described by bi- exponential fits, whereas the decay characteristics at 440 nm of urine samples was best explained by bi-exponential fits and, in some cases, by tri-exponential fits. However, the decay kinetics of both indoxyl sulfate and neopterin standards was well described by bi-exponential decays. Based on the fluorescence emission characteristics and statistical analysis, the fluorophores indoxyl sulfate, neopterin, and riboflavin may be considered as potential biomarkers for cervical cancer diagnosis.

Paper Details

Date Published: 19 March 2014
PDF: 10 pages
J. Biomed. Opt. 19(3) 037003 doi: 10.1117/1.JBO.19.3.037003
Published in: Journal of Biomedical Optics Volume 19, Issue 3
Show Author Affiliations
Ramu Rajasekaran, Anna Univ. Chennai (India)
Prakasa Rao Aruna, Anna Univ. Chennai (India)
Dornadula Koteeswaran, Meenakshi Ammal Dental College & Hospital (India)
Ganesan Bharanidharan, Anna Univ. Chennai (India)
Munusamy Baludavid, Government Arignar Anna Memorial Cancer Hospital (India)
Singaravelu Ganesan, Anna Univ. Chennai (India)

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