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Journal of Biomedical Optics • Open Access

Calibration-free absolute quantification of particle concentration by statistical analyses of photoacoustic signals in vivo

Paper Abstract

Currently, laser fluence calibration is typically required for quantitative measurement of particle concentration in photoacoustic imaging. Here, we present a calibration-free method to quantify the absolute particle concentration by statistically analyzing photoacoustic signals. The proposed method is based on the fact that Brownian motion induces particle count fluctuation in the detection volume. If the count of particles in the detection volume is assumed to follow the Poisson distribution, its expected value can be calculated by the photoacoustic signal mean and variance. We first derived a theoretical model for photoacoustic signals. Then, we applied our method to quantitative measurement of different concentrations of various particles, including red blood cells. Finally, we performed in vivo experiments to demonstrate the potential of our method in biological applications. The experimental results agreed well with the predictions from the theoretical model suggesting that our method can be used for noninvasive measurement of absolute particle concentrations in deep tissue without fluence calibration.

Paper Details

Date Published: 3 March 2014
PDF: 9 pages
J. Biomed. Opt. 19(3) 037001 doi: 10.1117/1.JBO.19.3.037001
Published in: Journal of Biomedical Optics Volume 19, Issue 3
Show Author Affiliations
Yong Zhou, Washington Univ. in St. Louis (United States)
Junjie Yao, Washington Univ. in St. Louis (United States)
Konstantin I. Maslov, Washington Univ. in St. Louis (United States)
Lihong V. Wang, Washington Univ. in St. Louis (United States)

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