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Journal of Biomedical Optics

Concentration dependence of optical clearing on the enhancement of laser-scanning optical-resolution photoacoustic microscopy imaging
Author(s): Qingliang Zhao; Lin Li; Qian Li; Xia Jiang; Qiushi Ren; Chuanqing Zhou
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Paper Abstract

Quantitative analysis of optical clearing effects (OCE) induced by hyperosmotic agents is very important to optical tissue clearing applications in biomedical diagnostic imaging and therapeutics. This study aims at investigating the effect of glycerol concentration on the laser-scanning optical-resolution photoacoustic microscopy (LSOR-PAM) imaging contrast and light penetration depth. The photoacoustic (PA) signal amplitude changes are evaluated as a function of the concentration of glycerol. The results reveal that the PA signal amplitudes are enhanced with the glycerol concentration increasing, and also show that higher concentration of glycerol produces better light penetration and OCE on a phantom. The PA signal amplitude increases only 8.1% for 20% glycerol, but for higher concentrations, the increases are 76% and 165% for 40% and 60% glycerol, respectively. This preliminary study demonstrates that application of glycerol as an optical contrast agent reduces the tissue scattering and is beneficial to PAM imaging and optical diagnosis in clinical dermatology.

Paper Details

Date Published: 26 March 2014
PDF: 8 pages
J. Biomed. Opt. 19(3) 036019 doi: 10.1117/1.JBO.19.3.036019
Published in: Journal of Biomedical Optics Volume 19, Issue 3
Show Author Affiliations
Qingliang Zhao, Shanghai Jiao Tong Univ. (China)
Lin Li, Shanghai Jiao Tong Univ. (China)
Qian Li, Shanghai Jiao Tong Univ. (China)
Xia Jiang, Shanghai Jiao Tong Univ. (China)
Qiushi Ren, Peking Univ. (China)
Chuanqing Zhou, Shanghai Jiao Tong Univ. (China)

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