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Journal of Biomedical Optics

High-speed multispectral confocal biomedical imaging
Author(s): Gary E. Carver; Sarah A. Locknar; William A. Morrison; V. Krishnan Ramanujan; Daniel L. Farkas
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Paper Abstract

A new approach for generating high-speed multispectral confocal images has been developed. The central concept is that spectra can be acquired for each pixel in a confocal spatial scan by using a fast spectrometer based on optical fiber delay lines. This approach merges fast spectroscopy with standard spatial scanning to create datacubes in real time. The spectrometer is based on a serial array of reflecting spectral elements, delay lines between these elements, and a single element detector. The spatial, spectral, and temporal resolution of the instrument is described and illustrated by multispectral images of laser-induced autofluorescence in biological tissues.

Paper Details

Date Published: 21 March 2014
PDF: 9 pages
J. Biomed. Opt. 19(3) 036016 doi: 10.1117/1.JBO.19.3.036016
Published in: Journal of Biomedical Optics Volume 19, Issue 3
Show Author Affiliations
Gary E. Carver, Omega Optical, Inc. (United States)
Sarah A. Locknar, Omega Optical, Inc. (United States)
William A. Morrison, Omega Optical, Inc. (United States)
V. Krishnan Ramanujan, Cedars-Sinai Medical Ctr. (United States)
Daniel L. Farkas, Spectral Molecular Imaging (United States)


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