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Journal of Biomedical Optics • Open Access

Spatially resolved optical and ultrastructural properties of colorectal and pancreatic field carcinogenesis observed by inverse spectroscopic optical coherence tomography
Author(s): Ji Yi; Andrew J. Radosevich; Yolanda Stypula-Cyrus; Nikhil N. Mutyal; Samira M. Azarin; Elizabeth Horcher; Michael J. Goldberg; Laura Bianchi; Shailesh Bajaj; Hemant K. Roy; Vadim Backman

Paper Abstract

Field carcinogenesis is the initial stage of cancer progression. Understanding field carcinogenesis is valuable for both cancer biology and clinical medicine. Here, we used inverse spectroscopic optical coherence tomography to study colorectal cancer (CRC) and pancreatic cancer (PC) field carcinogenesis. Depth-resolved optical and ultrastructural properties of the mucosa were quantified from histologically normal rectal biopsies from patients with and without colon adenomas (n=85 ) as well as from histologically normal peri-ampullary duodenal biopsies from patients with and without PC ([i]n=22 ). Changes in the epithelium and stroma in CRC field carcinogenesis were separately quantified. In both compartments, optical and ultra-structural alterations were consistent. Optical alterations included lower backscattering (μ b ) and reduced scattering (μ ′ s ) coefficients and higher anisotropy factor g . Ultrastructurally pronounced alterations were observed at length scales up to ∼450  nm , with the shape of the mass density correlation function having a higher shape factor D , thus implying a shift to larger length scales. Similar alterations were found in the PC field carcinogenesis despite the difference in genetic pathways and etiologies. We further verified that the chromatin clumping in epithelial cells and collagen cross-linking caused D to increase in vitro and could be among the mechanisms responsible for the observed changes in epithelium and stroma, respectively.

Paper Details

Date Published: 18 March 2014
PDF: 17 pages
J. Biomed. Opt. 19(3) 036013 doi: 10.1117/1.JBO.19.3.036013
Published in: Journal of Biomedical Optics Volume 19, Issue 3
Show Author Affiliations
Ji Yi, Northwestern Univ. (United States)
Andrew J. Radosevich, Northwestern Univ. (United States)
Yolanda Stypula-Cyrus, Northwestern Univ. (United States)
Nikhil N. Mutyal, Northwestern Univ. (United States)
Samira M. Azarin, Northwestern Univ. (United States)
Elizabeth Horcher, Northwestern Univ. (United States)
Michael J. Goldberg, NorthShore Univ. HealthSystem (United States)
Laura Bianchi, NorthShore Univ. HealthSystem (United States)
Shailesh Bajaj, NorthShore Univ. HealthSystem (United States)
Hemant K. Roy, Boston Medical Ctr. (United States)
Vadim Backman, Northwestern Univ. (United States)

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