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Journal of Biomedical Optics

R and G color component competition of RGB image decomposition as a criterion to register RBC agglutinates for blood group typing
Author(s): Valeri A. Doubrovski; Yulia A. Ganilova; Igor V. Zabenkov
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Paper Abstract

A new approach of the criterion assignment for registration of erythrocyte agglutinates to instrumentally determine blood group type is suggested. The criterion is based on comparison of R and G components of RGB decomposition of microscopy digital image taken for the blood-serum mixture sample. For the chosen experimental conditions, the minimal size (area) of RBC agglutinate to be registered by the criterion suggested is estimated theoretically. The proposed method was tested experimentally on the example of monitoring agglutinates in flow. The encouraging experimental results were obtained for improvement of the resolving power of the method; the optimal experimental conditions were revealed for maximum resolution. Though the suggested method was realized for dynamic (flow) blood group determination, it could also be applied for diagnostics in a stationary environment. This approach increases the reliability of RBC agglutinates registration and, hence, blood group typing. The results may be used to develop the apparatus for automated determination of human blood group.

Paper Details

Date Published: 17 March 2014
PDF: 3 pages
J. Biomed. Opt. 19(3) 036012 doi: 10.1117/1.JBO.19.3.036012
Published in: Journal of Biomedical Optics Volume 19, Issue 3
Show Author Affiliations
Valeri A. Doubrovski, Saratov State Medical Univ. (Russian Federation)
Yulia A. Ganilova, Saratov State Medical Univ. (Russian Federation)
Igor V. Zabenkov, Saratov State Medical Univ. (Russian Federation)

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