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Journal of Biomedical Optics • Open Access

In vivo therapy monitoring of experimental rheumatoid arthritis in rats using near-infrared fluorescence imaging
Author(s): Sonja Vollmer; Ines Gemeinhardt; Axel Vater; Beatrix Schnorr; Jörg Schnorr; Jan Voigt; Bernd Ebert

Paper Abstract

An in vivo near-infrared fluorescence (NIRF) imaging technique is described for therapy monitoring of ankle joints affected by collagen-induced arthritis, a model of human rheumatoid arthritis. Arthritis was induced in rats by intradermal injections of collagen and Freund’s incomplete adjuvant. For in vivo imaging, the nonspecific NIR dye tetrasulfocyanine (TSC) was used. Prior to and after treatment with a nonsteroidal anti-inflammatory drug, meloxicam, or analgesic drug, tramadol hydrochloride (which served as no-therapy control), normalized fluorescence intensities of each ankle joint were measured. Additionally, each ankle joint was characterized by clinical arthritis scoring and histopathology. Over a 3-week treatment period, a significant difference in disease progression between animals treated with meloxicam and tramadol hydrochloride was detected. A statistically significant improvement in ankle joint pathology from high- or moderate-grade to moderate- or low-grade upon meloxicam therapy, as determined by clinical evaluation, translated into a significant decrease in fluorescence intensity. In contrast, all arthritic joints of the no-therapy control group deteriorated to high-grade arthritis with high-fluorescence intensities in NIRF imaging.

Paper Details

Date Published: 17 March 2014
PDF: 8 pages
J. Biomed. Opt. 19(3) 036011 doi: 10.1117/1.JBO.19.3.036011
Published in: Journal of Biomedical Optics Volume 19, Issue 3
Show Author Affiliations
Sonja Vollmer, Bayer Schering Pharma AG (Germany)
Ines Gemeinhardt, Charité Universitätsmedizin Berlin (Germany)
Axel Vater, Bayer Schering Pharma AG (Germany)
Beatrix Schnorr, Charité Universitätsmedizin Berlin (Germany)
Jörg Schnorr, Charité Universitätsmedizin Berlin (Germany)
Jan Voigt, Physikalisch-Technische Bundesanstalt (Germany)
Bernd Ebert, Physikalisch-Technische Bundesanstalt (Germany)


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