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Journal of Biomedical Optics • Open Access

Diffraction phase microscopy: retrieving phase contours on living cells with a wavelet-based space-scale analysis
Author(s): Cristina Martinez-Torres; Lotfi Berguiga; Laura Streppa; Elise Boyer-Provera; Laurent Schaeffer; Juan Elezgaray; Alain Arneodo; Françoise Argoul

Paper Abstract

We propose a two-dimensional (2-D) space-scale analysis of fringe patterns collected from a diffraction phase microscope based on the 2-D Morlet wavelet transform. We show that the adaptation of a ridge detection method with anisotropic 2-D Morlet mother wavelets is more efficient for analyzing cellular and high refractive index contrast objects than Fourier filtering methods since it can separate phase from intensity modulations. We compare the performance of this ridge detection method on theoretical and experimental images of polymer microbeads and experimental images collected from living myoblasts.

Paper Details

Date Published: 10 March 2014
PDF: 12 pages
J. Biomed. Opt. 19(3) 036007 doi: 10.1117/1.JBO.19.3.036007
Published in: Journal of Biomedical Optics Volume 19, Issue 3
Show Author Affiliations
Cristina Martinez-Torres, Ecole Normale Supérieure de Lyon (France)
Lotfi Berguiga, Ecole Normale Supérieure de Lyon (France)
Laura Streppa, Ecole Normale Supérieure de Lyon (France)
Elise Boyer-Provera, Ecole Normale Supérieure de Lyon (France)
Laurent Schaeffer, Ecole Normale Supérieure de Lyon (France)
Juan Elezgaray, Univ. Bordeaux 1 (France)
Alain Arneodo, Ecole Normale Supérieure de Lyon (France)
Françoise Argoul, Ecole Normale Supérieure de Lyon (France)


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