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Journal of Biomedical Optics

Real-time label-free detection of dividing cells by means of lensfree video-microscopy
Author(s): Srikanth V. Kesavan; Fabrice P. Navarro Y Garcia; Mathilde Menneteau; Frédérique Mittler; Brigitte David-Watine; Nelly Dubrulle; Spencer L. Shorte; Bernard Chalmond; Jean-Marc Dinten; Cédric P. Allier
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Paper Abstract

Quantification of cell proliferation and monitoring its kinetics are essential in fields of research such as developmental biology, oncology, etc. Although several proliferation assays exist, monitoring cell proliferation kinetics remains challenging. We present a novel cell proliferation assay based on real-time monitoring of cell culture inside a standard incubator using a lensfree video-microscope, combined with automated detection of single cell divisions over a population of several thousand cells. Since the method is based on direct visualization of dividing cells, it is label-free, continuous, and not sample destructive. Kinetics of cell proliferation can be monitored from a few hours to several days. We compare our method to a standard assay, the EdU proliferation assay, and as proof of principle, we demonstrate concentration-dependent and time-dependent effect of actinomycin D—a cell proliferation inhibitor.

Paper Details

Date Published: 5 March 2014
PDF: 11 pages
J. Biomed. Opt. 19(3) 036004 doi: 10.1117/1.JBO.19.3.036004
Published in: Journal of Biomedical Optics Volume 19, Issue 3
Show Author Affiliations
Srikanth V. Kesavan, Commissariat à l'Énergie Atomique (France)
Fabrice P. Navarro Y Garcia, Commissariat à l'Énergie Atomique (France)
Mathilde Menneteau, Commissariat à l'Énergie Atomique (France)
Frédérique Mittler, Commissariat à l'Énergie Atomique (France)
Brigitte David-Watine, Institut Pasteur (France)
Nelly Dubrulle, Institut Pasteur (France)
Spencer L. Shorte, Institut Pasteur (France)
Bernard Chalmond, Ecole Normale Supérieure de Cachan (France)
Jean-Marc Dinten, Commissariat à l'Énergie Atomique (France)
Cédric P. Allier, Commissariat à l'Énergie Atomique (France)


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