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Journal of Biomedical Optics • Open Access

Differential diagnosis of nonmelanoma pigmented skin lesions based on harmonic generation microscopy
Author(s): Ming-Rung Tsai; Yu-Hsiang Cheng; Jau-Shiuh Chen; Yi-Shuan Sheen; Yi-Hua Liao; Chi-Kuang Sun

Paper Abstract

In vivo harmonic generation microscopy (HGM) has been applied successfully in healthy human skin and can achieve a submicron resolution, similar to histopathologic examination, even at a penetration depth up to 270 μm. This study aims to investigate the clinical applicability of HGM imaging for differential diagnosis of nonmelanoma pigmented skin lesions. A total of 42 pigmented skin tumors, including pigmented basal cell carcinoma, melanocytic nevus, and seborrheic keratosis were evaluated by HGM ex vivo or in vivo. Based on the standard histopathologic characteristics, we established the corresponding HGM imaging criteria for each pigmented tumor. Diagnostic performance of HGM for differentiating nonmelanoma pigmented skin tumors was evaluated through the observers’ direct general assessment (overall evaluation) or the presence of two imaging criteria with the highest sensitivity and specificity (major criteria evaluation). Our results show that, based on the direct general assessment, the sensitivity is 92% [95% confidence interval (CI): 67 to 97%] and the specificity is 96% (95% CI: 83 to 99%); by major criteria evaluation, 94% sensitivity (95% CI: 70 to 99%) and 100% specificity (95% CI: 87 to 100%) are achieved. Our study indicates that HGM serves as a promising histopathological examination tool for noninvasive differential diagnostics of nonmelanoma pigmented skin tumors.

Paper Details

Date Published: 3 March 2014
PDF: 9 pages
J. Biomed. Opt. 19(3) 036001 doi: 10.1117/1.JBO.19.3.036001
Published in: Journal of Biomedical Optics Volume 19, Issue 3
Show Author Affiliations
Ming-Rung Tsai, National Taiwan Univ. (Taiwan)
Yu-Hsiang Cheng, National Taiwan Univ. (Taiwan)
Jau-Shiuh Chen, National Taiwan Univ. Hospital (Taiwan)
Yi-Shuan Sheen, National Taiwan Univ. Hospital (Taiwan)
Yi-Hua Liao, National Taiwan Univ. (Taiwan)
Chi-Kuang Sun, National Taiwan Univ. (Taiwan)

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