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Journal of Biomedical Optics

Photobiological effect of low-level laser irradiation in bovine embryo production system
Author(s): Carlos Alexandre Soares; Kelly Annes; Thiago R. Dreyer; Taciana D. Magrini; Mayra T. Sonoda; Herculano S. da Silva Martinho; Marcilio Nichi; Mayra Elena Ortiz d'Avila Assumpcao; Marcella P. Milazzotto
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Paper Abstract

The objectives of this study were to evaluate the effect of low-level laser irradiation (LLLI) on bovine oocyte and granulosa cells metabolism during in vitro maturation (IVM) and further embryo development. Cumulus-oocytes complexes (COCs) were subjected (experimental group) or not (control group) to irradiation with LLLI in a 633-nm wavelength and 1  J/cm 2 fluency. The COCs were evaluated after 30 min, 8, 16, and 24 h of IVM. Cumulus cells were evaluated for cell cycle status, mitochondrial activity, and viability (flow cytometry). Oocytes were assessed for meiotic progression status (nuclear staining), cell cycle genes content [real-time polymerase chain reaction (PCR)], and signal transduction status (western blot). The COCs were also in vitro fertilized, and the cleavage and blastocyst rates were assessed. Comparisons among groups were statistically performed with 5% significance level. For cumulus cells, a significant increase in mitochondrial membrane potential and the number of cells progressing through the cycle could be observed. Significant increases on cyclin B and cyclin-dependent kinase (CDK4) levels were also observed. Concerning the oocytes, a significantly higher amount of total mitogen-activated protein kinase was found after 8 h of irradiation, followed by a decrease in all cell cycle genes transcripts, exception made for the CDK4. However, no differences were observed in meiotic progression or embryo production. In conclusion, LLLI is an efficient tool to modulate the granulosa cells and oocyte metabolism.

Paper Details

Date Published: 21 March 2014
PDF: 10 pages
J. Biomed. Opt. 19(3) 035006 doi: 10.1117/1.JBO.19.3.035006
Published in: Journal of Biomedical Optics Volume 19, Issue 3
Show Author Affiliations
Carlos Alexandre Soares, UFABC (Brazil)
Kelly Annes, UFABC (Brazil)
Thiago R. Dreyer, UFABC (Brazil)
Taciana D. Magrini, UFABC (Brazil)
Mayra T. Sonoda, UFABC (Brazil)
Herculano S. da Silva Martinho, UFABC (Brazil)
Marcilio Nichi, Univ. de São Paulo (Brazil)
Mayra Elena Ortiz d'Avila Assumpcao, Univ. de São Paulo (Brazil)
Marcella P. Milazzotto, UFABC (Brazil)


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