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Journal of Biomedical Optics

Ultrasensitive opto-microfluidic immunosensor integrating gold nanoparticle–enhanced chemiluminescence and highly stable organic photodetector
Author(s): Nuno Miguel Matos Pires; Tao Dong
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Paper Abstract

The expensive fabrication of current opto-microfluidic sensors is a barrier to the successful adoption of these devices in point-of-care testing. This work reports a simple inexpensive opto-microfluidic device incorporating a poly(dimethylsiloxane)-glass hybrid microfluidic chip modified with gold nanoparticles and a high-detectivity, high-stability organic photodetector. The enhancing effect of the gold nanoparticles on horseradish peroxidase-luminol-H 2 O 2 chemiluminescence was exploited in rapid single-analyte immunoassays. The limit of detection for 17-β estradiol was 2.5  pg/ml , which is ∼200 times more sensitive than previously reported chemiluminescent immunosensors employing other organic photodetectors. Detection was also demonstrated in complex media, including natural water and blood serum.

Paper Details

Date Published: 10 March 2014
PDF: 4 pages
J. Biomed. Opt. 19(3) 030504 doi: 10.1117/1.JBO.19.3.030504
Published in: Journal of Biomedical Optics Volume 19, Issue 3
Show Author Affiliations
Nuno Miguel Matos Pires, Vestfold Univ. College (Norway)
Tao Dong, Vestfold Univ. College (Norway)

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