Journal of Biomedical Optics • Open AccessCoherent Raman Scattering Microscopy
Reviewed by Alberto Diaspro, SPIE Fellow, Istituto Italiano di Tecnologia, Genova, Italy Research and development of modern optical microscopes are guided by several aspects, from resolution obsession to imaging of living specimens, from a low-level of perturbation to long-term experiments on biological systems. One of the most outstanding goals is the possibility of performing at the highest current levels, including 4-D (x,y,z,t) nanoscopy/superresolution, label-free imaging. Coherent Raman scattering (CRS) microscopy represents a key label-free imaging method for studying living cells and tissues at a high time rate. Ji-Xin Cheng and Xiaoliang Sunney Xie, two of the most representative scientists in this area, assembled the perfect book for disseminating the method. Their experience as scientists and authors and their robustness and consideration in the field allowed them to edit a great book that, starting from the foundation of CRS microscopy, covers the field in a comprehensive and exhaustive way.