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Journal of Biomedical Optics • Open Access

Mueller matrix three-dimensional directional imaging of collagen fibers
Author(s): Pål G. Ellingsen; Lars Martin S. Aas; Vegard S. Hagen; Rajesh Kumar; Magnus B. Lilledahl; Morten Kildemo

Paper Abstract

A method for measuring three-dimensional (3-D) direction images of collagen fibers in biological tissue is presented. Images of the 3-D directions are derived from the measured transmission Mueller matrix images (MMIs), acquired at different incidence angles, by taking advantage of the form birefringence of the collagen fibers. The MMIs are decomposed using the recently developed differential decomposition, which is more suited to biological tissue samples than the common polar decomposition method. Validation of the 3-D direction images was performed by comparing them with images from second-harmonic generation microscopy. The comparison found a good agreement between the two methods. It is envisaged that 3-D directional imaging could become a useful tool for understanding the collagen framework for fibers smaller than the diffraction limit.

Paper Details

Date Published: 6 February 2014
PDF: 7 pages
J. Biomed. Opt. 19(2) 026002 doi: 10.1117/1.JBO.19.2.026002
Published in: Journal of Biomedical Optics Volume 19, Issue 2
Show Author Affiliations
Pål G. Ellingsen, Norwegian Univ. of Science and Technology (Norway)
Lars Martin S. Aas, Norwegian Univ. of Science and Technology (Norway)
Vegard S. Hagen, Norwegian Univ. of Science and Technology (Norway)
Rajesh Kumar, Norwegian Univ. of Science and Technology (Norway)
Magnus B. Lilledahl, Norwegian Univ. of Science and Technology (Norway)
Morten Kildemo, Norwegian Univ. of Science and Technology (Norway)


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