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Journal of Biomedical Optics

Fluorescent probes concentration estimation <italic<in vitro</italic< and <italic<ex vivo</italic< as a model for early detection of Alzheimer’s disease
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Paper Abstract

The pathogenic process of Alzheimer’s disease (AD) begins years before clinical diagnosis. Here, we suggest a method that may detect AD several years earlier than current exams. The method is based on previous reports that relate the concentration ratio of biomarkers (amyloid-beta and tau) in the cerebrospinal fluid (CSF) to the development of AD. Our method replaces the lumbar puncture process required for CSF drawing by using fluorescence measurements. The system uses an optical fiber coupled to a laser source and a detector. The laser radiation excites two fluorescent probes which may bond to the CSF biomarkers. Their concentration ratio is extracted from the fluorescence intensities and can be used for future AD detection. First, we present a theoretical model for fluorescence concentration ratio estimation. The method’s feasibility was validated using Monte Carlo simulations. Its accuracy was then tested using multilayered tissue phantoms simulating the epidural fat, CSF, and bone. These phantoms have various optical properties, thicknesses, and fluorescence concentrations in order to simulate human anatomy variations and different fiber locations. The method was further tested using <italic<ex vivo</italic< chicken tissue. The average errors of the estimated concentration ratios were low both <italic<in vitro</italic< (4.4%) and <italic<ex vivo</italic< (10.9%), demonstrating high accuracy.

Paper Details

Date Published: 29 December 2014
PDF: 9 pages
J. Biomed. Opt. 19(12) 127007 doi: 10.1117/1.JBO.19.12.127007
Published in: Journal of Biomedical Optics Volume 19, Issue 12
Show Author Affiliations
Osnat Harbater, Tel Aviv Univ. (Israel)
Israel Gannot, Tel Aviv Univ. (Israel)
Johns Hopkins Univ. (United States)


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