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Journal of Biomedical Optics

Combined lineage mapping and gene expression profiling of embryonic brain patterning using ultrashort pulse microscopy and image registration
Author(s): Holly C. Gibbs; Colin R. Dodson; Yuqiang Bai; Arne C. Lekven; Alvin T. Yeh
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Paper Abstract

During embryogenesis, presumptive brain compartments are patterned by dynamic networks of gene expression. The spatiotemporal dynamics of these networks, however, have not been characterized with sufficient resolution for us to understand the regulatory logic resulting in morphogenetic cellular behaviors that give the brain its shape. We have developed a new, integrated approach using ultrashort pulse microscopy [a high-resolution, two-photon fluorescence (2PF)-optical coherence microscopy (OCM) platform using 10-fs pulses] and image registration to study brain patterning and morphogenesis in zebrafish embryos. As a demonstration, we used time-lapse 2PF to capture midbrain-hindbrain boundary morphogenesis and a

Paper Details

Date Published: 24 December 2014
PDF: 14 pages
J. Biomed. Opt. 19(12) 126016 doi: 10.1117/1.JBO.19.12.126016
Published in: Journal of Biomedical Optics Volume 19, Issue 12
Show Author Affiliations
Holly C. Gibbs, Texas A&M Univ. (United States)
Colin R. Dodson, Texas A&M Univ. (United States)
Yuqiang Bai, Texas A&M Univ. (United States)
Arne C. Lekven, Texas A&M Univ. (United States)
Alvin T. Yeh, Texas A&M Univ. (United States)


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