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Journal of Biomedical Optics • Open Access

Trimodal color-fluorescence-polarization endoscopy aided by a tumor selective molecular probe accurately detects flat lesions in colitis-associated cancer
Author(s): Tauseef Charanya; Timothy York; Sharon Bloch; Gail Sudlow; Kexian Liang; Missael Garcia; Walter J. Akers; Deborah Rubin; Viktor Gruev; Samuel Achilefu

Paper Abstract

Colitis-associated cancer (CAC) arises from premalignant flat lesions of the colon, which are difficult to detect with current endoscopic screening approaches. We have developed a complementary fluorescence and polarization reporting strategy that combines the unique biochemical and physical properties of dysplasia and cancer for real-time detection of these lesions. Using azoxymethane-dextran sodium sulfate (AOM-DSS) treated mice, which recapitulates human CAC and dysplasia, we show that an octapeptide labeled with a near-infrared (NIR) fluorescent dye selectively identified all precancerous and cancerous lesions. A new thermoresponsive sol-gel formulation allowed topical application of the molecular probe during endoscopy. This method yielded high contrast-to-noise ratios (CNR) between adenomatous tumors (20.6±1.65) and flat lesions (12.1±1.03) and surrounding uninvolved colon tissue versus CNR of inflamed tissues (1.62±0.41). Incorporation of nanowire-filtered polarization imaging into NIR fluorescence endoscopy shows a high depolarization contrast in both adenomatous tumors and flat lesions in CAC, reflecting compromised structural integrity of these tissues. Together, the real-time polarization imaging provides real-time validation of suspicious colon tissue highlighted by molecular fluorescence endoscopy.

Paper Details

Date Published: 4 December 2014
PDF: 14 pages
J. Biomed. Opt. 19(12) 126002 doi: 10.1117/1.JBO.19.12.126002
Published in: Journal of Biomedical Optics Volume 19, Issue 12
Show Author Affiliations
Tauseef Charanya, Washington Univ. School of Medicine in St. Louis (United States)
Timothy York, Washington Univ. in St. Louis (United States)
Sharon Bloch, Washington Univ. in St. Louis (United States)
Gail Sudlow, Washington Univ. School of Medicine in St. Louis (United States)
Kexian Liang, Washington Univ. School of Medicine in St. Louis (United States)
Missael Garcia, Washington Univ. in St. Louis (United States)
Walter J. Akers, Washington Univ. School of Medicine in St. Louis (United States)
Deborah Rubin, Washington Univ. in St. Louis (United States)
Viktor Gruev, Washington Univ. in St. Louis (United States)
Samuel Achilefu, Washington Univ. School of Medicine in St. Louis (United States)


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