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Journal of Biomedical Optics

In-fiber photo-immobilization of a bioactive surface
Author(s): Elizabeth Lee; Derrick Yong; Xia Yu; Hao Li; Chi Chiu Chan
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Paper Abstract

We demonstrate the first in-fiber light-induced bioactive biotin-functionalization via photobleaching fluorophore-conjugated biotin. Photobleaching the fluorophores generated free radicals that bind to the albumin-passivated inner surface of pure silica photonic crystal fiber. The subsequent attachment of dye-conjugated streptavidin to the bound biotin qualified the photo-immobilization process and demonstrated a potential for the construction of in-fiber macromolecular assemblies or multiplexes. Compared with other in-fiber bioactive coating methods, the proposed light-induced technique requires only a low-power light source, without the need for additional preactivation steps or toxic chemical reagents. This method, hence, enables a simple and compact implementation for potential biomedical applications.

Paper Details

Date Published: 18 December 2014
PDF: 3 pages
J. Biomed. Opt. 19(12) 120502 doi: 10.1117/1.JBO.19.12.120502
Published in: Journal of Biomedical Optics Volume 19, Issue 12
Show Author Affiliations
Elizabeth Lee, Precision Measurements Group (Singapore)
Nanyang Technological Univ. (Singapore)
Derrick Yong, Singapore Institute of Manufacturing Technology (Singapore)
Nanyang Technological Univ. (Singapore)
Xia Yu, Singapore Institute of Manufacturing Technology (Singapore)
Hao Li, Singapore Institute of Manufacturing Technology (Singapore)
Chi Chiu Chan, Nanyang Technological Univ. (Singapore)


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