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Journal of Biomedical Optics

Delayed luminescence to monitor programmed cell death induced by berberine on thyroid cancer cells
Author(s): Agata Scordino; Agata Campisi; Rosaria Grasso; Roberta Bonfanti; Marisa Gulino; Liliana Iauk; Rosalba Parenti; Francesco Musumeci
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Paper Abstract

Correlation between apoptosis and UVA-induced ultraweak photon emission delayed luminescence (DL) from tumor thyroid cell lines was investigated. In particular, the effects of berberine, an alkaloid that has been reported to have anticancer activities, on two cancer cell lines were studied. The FTC-133 and 8305C cell lines, as representative of follicular and anaplastic thyroid human cancer, respectively, were chosen. The results show that berberine is able to arrest cell cycle and activate apoptotic pathway as shown in both cell lines by deoxyribonucleic acid fragmentation, caspase-3 cleavage, p53 and p27 protein overexpression. In parallel, changes in DL spectral components after berberine treatment support the hypothesis that DL from human cells originates mainly from mitochondria, since berberine acts especially at the mitochondrial level. The decrease of DL blue component for both cell lines could be related to the decrease of intra-mitochondrial nicotinamide adenine dinucleotide and may be a hallmark of induced apoptosis. In contrast, the response in the red spectral range is different for the two cell lines and may be ascribed to a different iron homeostasis.

Paper Details

Date Published: 13 November 2014
PDF: 10 pages
J. Biomed. Opt. 19(11) 117005 doi: 10.1117/1.JBO.19.11.117005
Published in: Journal of Biomedical Optics Volume 19, Issue 11
Show Author Affiliations
Agata Scordino, Univ. degli Studi di Catania (Italy)
Instituto Nazionale di Fisica Nucleare (Italy)
Agata Campisi, Univ. degli Studi di Catania (Italy)
Rosaria Grasso, Istituto Nazionale di Fisica Nucleare (Italy)
Roberta Bonfanti, Univ. degli Studi di Catania (Italy)
Marisa Gulino, Istituto Nazionale di Fisica Nucleare (Italy)
“Kore” Univ. (Italy)
Liliana Iauk, Univ. degli Studi di Catania (Italy)
Rosalba Parenti, Univ. degli Studi di Catania (Italy)
Francesco Musumeci, Univ. degli Studi di Catania (Italy)
Istituto Nazionale di Fisica Nucleare (Italy)

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