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Journal of Biomedical Optics • Open Access

Multiphoton gradient index endoscopy for evaluation of diseased human prostatic tissue <italic<ex vivo</italic<
Author(s): David M. Huland; Manu Jain; Dimitre G. Ouzounov; Brian D. Robinson; Diana S. Harya; Maria M. Shevchuk; Paras Singhal; Chris Xu; Ashutosh K. Tewari

Paper Abstract

Multiphoton microscopy can instantly visualize cellular details in unstained tissues. Multiphoton probes with clinical potential have been developed. This study evaluates the suitability of multiphoton gradient index (GRIN) endoscopy as a diagnostic tool for prostatic tissue. A portable and compact multiphoton endoscope based on a 1-mm diameter, 8-cm length GRIN lens system probe was used. Fresh <italic<ex vivo</italic< samples were obtained from 14 radical prostatectomy patients and benign and malignant areas were imaged and correlated with subsequent H&E sections. Multiphoton GRIN endoscopy images of unfixed and unprocessed prostate tissue at a subcellular resolution are presented. We note several differences and identifying features of benign versus low-grade versus high-grade tumors and are able to identify periprostatic tissues such as adipocytes, periprostatic nerves, and blood vessels. Multiphoton GRIN endoscopy can be used to identify both benign and malignant lesions in <italic<ex vivo</italic< human prostate tissue and may be a valuable diagnostic tool for real-time visualization of suspicious areas of the prostate.

Paper Details

Date Published: 21 November 2014
PDF: 7 pages
J. Biomed. Opt. 19(11) 116011 doi: 10.1117/1.JBO.19.11.116011
Published in: Journal of Biomedical Optics Volume 19, Issue 11
Show Author Affiliations
David M. Huland, Cornell Univ. (United States)
Manu Jain, New York-Presbyterian Hospital (United States)
Dimitre G. Ouzounov, Cornell Univ. (United States)
Brian D. Robinson, New York Presbyterian Hospital (United States)
Diana S. Harya, Cornell Univ. College of Veterinary Medicine (United States)
Maria M. Shevchuk, Weill Cornell Medical College (United States)
Paras Singhal, New York Presbyterian Hospital (United States)
Chris Xu, Cornell Univ. (United States)
Ashutosh K. Tewari, New York-Presbyterian Hospital (United States)


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