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Journal of Biomedical Optics

Depth-enhanced fluorescence imaging using masked detection of structured illumination
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Paper Abstract

There is a growing interest in imaging fluorescence contrast at depth within living tissues over wide fields of view and in real time. Most methods used to date to improve depth detection of fluorescence information involve acquisition of multiple images, postprocessing of the data using a light propagation model, and are capable of providing either depth-sectioned or tomographic fluorescence information. We introduce a method, termed masked detection of structured illumination, that allows the enhancement of fluorescence imaging at depth without postprocessing. This method relies on the scanning of a collimated beam onto a turbid medium and the physical masking of the point spread function on the detection arm before acquisition on a CCD camera. By preferentially collecting diffuse photons at a chosen source-detector range, this method enhances fluorescence information at depth and has the potential to form images without postprocessing and in real time.

Paper Details

Date Published: 14 November 2014
PDF: 7 pages
J. Biomed. Opt. 19(11) 116008 doi: 10.1117/1.JBO.19.11.116008
Published in: Journal of Biomedical Optics Volume 19, Issue 11
Show Author Affiliations
Joseph P. Angelo, Beth Israel Deaconess Medical Ctr. (United States)
Boston Univ. (United States)
Vivek Venugopal, Beth Israel Deaconess Medical Ctr. (United States)
Frederic Fantoni, MINATEC (France)
Vincent Poher, CEA-LETI (France)
Irving J. Bigio, Boston Univ. (United States)
Lionel Hervé, MINATEC (France)
Jean-Marc Dinten, MINATEC (France)
Sylvain Gioux, Beth Israel Deaconess Medical Ctr. (United States)

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