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Journal of Biomedical Optics • Open Access

Morphological phenotyping of mouse hearts using optical coherence tomography
Author(s): Michelle Cua; Eric Lin; Ling Lee; Xiaoye Sheng; Kevin S. K. Wong; Glen F. Tibbits; Mirza Faisal Beg; Marinko V. Sarunic

Paper Abstract

Transgenic mouse models have been instrumental in the elucidation of the molecular mechanisms behind many genetically based cardiovascular diseases such as Marfan syndrome (MFS). However, the characterization of their cardiac morphology has been hampered by the small size of the mouse heart. In this report, we adapted optical coherence tomography (OCT) for imaging fixed adult mouse hearts, and applied tools from computational anatomy to perform morphometric analyses. The hearts were first optically cleared and imaged from multiple perspectives. The acquired volumes were then corrected for refractive distortions, and registered and stitched together to form a single, high-resolution OCT volume of the whole heart. From this volume, various structures such as the valves and myofibril bundles were visualized. The volumetric nature of our dataset also allowed parameters such as wall thickness, ventricular wall masses, and luminal volumes to be extracted. Finally, we applied the entire acquisition and processing pipeline in a preliminary study comparing the cardiac morphology of wild-type mice and a transgenic mouse model of MFS.

Paper Details

Date Published: 13 November 2014
PDF: 8 pages
J. Biomed. Opt. 19(11) 116007 doi: 10.1117/1.JBO.19.11.116007
Published in: Journal of Biomedical Optics Volume 19, Issue 11
Show Author Affiliations
Michelle Cua, Simon Fraser Univ. (Canada)
Eric Lin, Simon Fraser Univ. (Canada)
Ling Lee, Simon Fraser Univ. (Canada)
Child and Family Research Institute (Canada)
Xiaoye Sheng, Child & Family Research Institute (Canada)
Kevin S. K. Wong, Simon Fraser Univ. (Canada)
Glen F. Tibbits, Simon Fraser Univ. (Canada)
Child & Family Research Institute (Canada)
Mirza Faisal Beg, Simon Fraser Univ. (Canada)
Marinko V. Sarunic, Simon Fraser Univ. (Canada)

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