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Journal of Biomedical Optics

<italic<In vivo</italic< label-free quantification of liver microcirculation using dual-modality microscopy
Author(s): Jie Yan; Yuzhan Kang; Shuoyu Xu; Lee-Ling S. Ong; Shuangmu Zhuo; Ralph M. Bunte; Nanguang Chen; H. Harry Asada; Peter T. C. So; Ian R. Wanless; Hanry Yu
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Paper Abstract

Microcirculation lesion is a common symptom of chronic liver diseases in the form of vasculature deformation and circulation alteration. In acute to chronic liver diseases such as biliary atresia, microcirculation lesion can have an early onset. Detection of microcirculation lesion is meaningful for studying the progression of liver disease. We have combined wide-field fluorescence microscopy and a laser speckle contrast technique to characterize hepatic microcirculation <italic<in vivo</italic< without labeling in a bile-duct ligation rat fibrosis model of biliary atresia. Through quantitative image analysis of four microcirculation parameters, we observed significant microcirculation lesion in the early to middle stages of fibrosis. This bimodal imaging method is useful to assess hepatic microcirculation lesion for the study of liver diseases.

Paper Details

Date Published: 11 November 2014
PDF: 6 pages
J. Biomed. Opt. 19(11) 116006 doi: 10.1117/1.JBO.19.11.116006
Published in: Journal of Biomedical Optics Volume 19, Issue 11
Show Author Affiliations
Jie Yan, BioSystems and Micromechanics (BioSyM) IRG (Singapore)
Institute of Bioengineering and Nanotechnology (Singapore)
National Univ. of Singapore (Singapore)
Yuzhan Kang, BioSystems and Micromechanics (BioSyM) IRG (Singapore)
Zhujiang Hospital of Southern Medical Univ. (China)
Shuoyu Xu, BioSystems and Micromechanics (BioSyM) IRG (Singapore)
Institute of Bioengineering and Nanotechnology (Singapore)
Lee-Ling S. Ong, SMART-Singapore MIT Alliance for Research & Technology (Singapore)
Shuangmu Zhuo, BioSystems and Micromechanics (BioSyM) IRG (Singapore)
Fujian Normal Univ. (China)
Ralph M. Bunte, Duke-NUS Graduate Medical School (Singapore)
Nanguang Chen, National Univ. of Singapore (Singapore)
H. Harry Asada, BioSystems and Micromechanics (BioSyM) IRG (Singapore)
Massachusetts Institute of Technology (United States)
Peter T. C. So, BioSystems and Micromechanics (BioSyM) IRG (Singapore)
Massachusetts Institute of Technology (United States)
(United States)
Ian R. Wanless, Dalhousie Univ. (Canada)
Hanry Yu, National Univ. of Singapore (Singapore)
Institute of Bioengineering and Nanotechnology (Singapore)
BioSystems and Micromechanics (BioSyM) IRG (Singapore)


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