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Journal of Biomedical Optics

Dynamic fluorescence lifetime imaging based on acousto-optic deflectors
Author(s): Wei Yan; Xiao Peng; Jing Qi; Jian Gao; Shunping Fan; Qi Wang; Junle Qu; Hanben Niu
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Paper Abstract

We report a dynamic fluorescence lifetime imaging (D-FLIM) system that is based on a pair of acousto-optic deflectors for the random regions of interest (ROI) study in the sample. The two-dimensional acousto-optic deflector devices are used to rapidly scan the femtosecond excitation laser beam across the sample, providing specific random access to the ROI. Our experimental results using standard fluorescent dyes in live cancer cells demonstrate that the D-FLIM system can dynamically monitor the changing process of the microenvironment in the ROI in live biological samples.

Paper Details

Date Published: 6 November 2014
PDF: 5 pages
J. Biomed. Opt. 19(11) 116004 doi: 10.1117/1.JBO.19.11.116004
Published in: Journal of Biomedical Optics Volume 19, Issue 11
Show Author Affiliations
Wei Yan, Shenzhen Univ. (China)
Xiao Peng, Shenzhen Univ. (China)
Jing Qi, Shenzhen Univ. (China)
Jian Gao, Shenzhen Univ. (China)
Shunping Fan, Shenzhen Univ. (China)
Qi Wang, Shenzhen Univ. (China)
Junle Qu, Shenzhen Univ. (China)
Hanben Niu, Shenzhen Univ. (China)

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