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Journal of Biomedical Optics

Digital holographic microscopy for longitudinal volumetric imaging of growth and treatment response in three-dimensional tumor models
Author(s): Yuyu Li; Ljubica Petrovic; Jeffrey La; Jonathan P. Celli; Chandra S. Yelleswarapu
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Paper Abstract

We report the use of digital holographic microscopy (DHM) as a viable microscopy approach for quantitative, nondestructive longitudinal imaging of <italic<in vitro</italic< three-dimensional (3-D) tumor models. Following established methods, we prepared 3-D cultures of pancreatic cancer cells in overlay geometry on extracellular matrix beds and obtained digital holograms at multiple time points throughout the duration of growth. The holograms were digitally processed and the unwrapped phase images were obtained to quantify the nodule thickness over time under normal growth and in cultures subject to chemotherapy treatment. In this manner, total nodule volumes are rapidly estimated and demonstrated here to show contrasting time-dependent changes during growth and in response to treatment. This work suggests the utility of DHM to quantify changes in 3-D structure over time and suggests the further development of this approach for time-lapse monitoring of 3-D morphological changes during growth and in response to treatment that would otherwise be impractical to visualize.

Paper Details

Date Published: 3 November 2014
PDF: 8 pages
J. Biomed. Opt. 19(11) 116001 doi: 10.1117/1.JBO.19.11.116001
Published in: Journal of Biomedical Optics Volume 19, Issue 11
Show Author Affiliations
Yuyu Li, Univ. of Massachusetts Boston (United States)
Ljubica Petrovic, Univ. of Massachusetts Boston (United States)
Jeffrey La, Univ. of Massachusetts Boston (United States)
Jonathan P. Celli, Univ. of Massachusetts Boston (United States)
Chandra S. Yelleswarapu, Univ. of Massachusetts Boston (United States)

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