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Journal of Biomedical Optics

Surface plasmon resonance–based infrared biosensor for cell studies with simultaneous control
Author(s): Alexander Zilbershtein; Amir Bein; Vladislav G. Lirtsman; Betty Schwartz; Michael Golosovsky; Dan Davidov
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Paper Abstract

We report a label-free infrared surface plasmon biosensor with a double-chamber flow cell for continuous monitoring of morphological changes in cell culture exposed to various stimuli. In this technique, the monolayer of cultured cells is divided into two halves by a barrier, allowing the treatment of one half while the other serves as control. We demonstrate the advantages of this setup in test experiments that track kinetics of the IEC-18 cell layer response to variations in extracellular Ca2+ concentration. The sensitivity of the presented method was found to be an order of magnitude higher compared to the single-chamber biosensor.

Paper Details

Date Published: 27 June 2014
PDF: 8 pages
J. Biomed. Opt. 19(11) 111608 doi: 10.1117/1.JBO.19.11.111608
Published in: Journal of Biomedical Optics Volume 19, Issue 11
Show Author Affiliations
Alexander Zilbershtein, The Hebrew Univ. of Jerusalem (Israel)
Amir Bein, The Hebrew Univ. of Jerusalem (Israel)
Vladislav G. Lirtsman, The Hebrew Univ. of Jerusalem (Israel)
Betty Schwartz, The Hebrew Univ. of Jerusalem (Israel)
Michael Golosovsky, The Hebrew Univ. of Jerusalem (Israel)
Dan Davidov, The Hebrew Univ. of Jerusalem (Israel)

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