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Journal of Biomedical Optics • Open Access

Quantum cascade laser–based hyperspectral imaging of biological tissue
Author(s): Niels Kröger; Alexander Egl; Maria Engel; Norbert Gretz; Katharina Haase; Iris Herpich; Bettina Kränzlin; Sabine Neudecker; Annemarie Pucci; Arthur Schönhals; Jochen Vogt; Wolfgang Petrich

Paper Abstract

The spectroscopy of analyte-specific molecular vibrations in tissue thin sections has opened up a path toward histopathology without the need for tissue staining. However, biomedical vibrational imaging has not yet advanced from academic research to routine histopathology due to long acquisition times for the microscopic hyperspectral images and/or cost and availability of the necessary equipment. Here we show that the combination of a fast-tuning quantum cascade laser with a microbolometer array detector allows for a rapid image acquisition and bares the potential for substantial cost reduction. A 3.1×2.8  mm2 unstained thin section of mouse jejunum has been imaged in the 9.2 to 9.7 μm wavelength range (spectral resolution ∼1  cm−1) within 5 min with diffraction limited spatial resolution. The comparison of this hyperspectral imaging approach with standard Fourier transform infrared imaging or mapping of the identical sample shows a reduction in acquisition time per wavenumber interval and image area by more than one or three orders of magnitude, respectively.

Paper Details

Date Published: 26 June 2014
PDF: 6 pages
J. Biomed. Opt. 19(11) 111607 doi: 10.1117/1.JBO.19.11.111607
Published in: Journal of Biomedical Optics Volume 19, Issue 11
Show Author Affiliations
Niels Kröger, Ruprecht-Karls-Univ. Heidelberg (Germany)
Alexander Egl, Ruprecht-Karls-Univ. Heidelberg (Germany)
Maria Engel, Ruprecht-Karls-Univ. Heidelberg (Germany)
Norbert Gretz, Ruprecht-Karls-Univ. Heidelberg (Germany)
Katharina Haase, Ruprecht-Karls-Univ. Heidelberg (Germany)
Iris Herpich, Ruprecht-Karls-Univ. Heidelberg (Germany)
Bettina Kränzlin, Ruprecht-Karls-Univ. Heidelberg (Germany)
Sabine Neudecker, Ruprecht-Karls-Univ. Heidelberg (Germany)
Annemarie Pucci, Ruprecht-Karls-Univ. Heidelberg (Germany)
Arthur Schönhals, University of Heidelberg (Germany)
Jochen Vogt, Ruprecht-Karls-Univ. Heidelberg (Germany)
Wolfgang Petrich, Ruprecht-Karls-Univ. Heidelberg (Germany)

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