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Journal of Biomedical Optics

Different doses of low-level laser irradiation modulate the in vitro response of osteoblast-like cells
Author(s): Serena Incerti Parenti; Luigi Checchi; Milena Fini; Matilde Tschon
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Paper Abstract

Because osteoblasts play a key role in bone remodeling and the influence of low-level laser therapy on this process is not clear, Saos-2 human osteoblast-like cells were irradiated by a gallium–aluminum–arsenide diode laser (915 nm) for 10, 48, 96, 193, and 482 s using doses 1, 5, 10, 20, and 50  J/cm2, respectively. A control group was not irradiated. Morphology, viability, and cytotoxicity analyses were carried out after 1 hr, 1 day, and 3 days. Deoxyribose nucleic acid (DNA) content and release of vascular endothelial growth factor (VEGF), receptor activator of nuclear factor kappa B ligand (RANKL), and osteoprotegerin (OPG) were evaluated. Viability was modulated by laser irradiation in a dose-dependent manner, with 10  J/cm2 inducing a biostimulatory response and 20 to 50  J/cm2 determining a bioinhibitory and cytotoxic effect. Accordingly, DNA content was generally increased for the 10  J/cm2 dose and decreased for the 50  J/cm2 dose. A rapid and transitory trend toward increased RANKL/OPG ratio and a tendency toward a delayed increase in VEGF release for doses of 1 to 10  J/cm2 was found. Further investigations using the biostimulatory dose of 10  J/cm2 emerged from this study are needed to establish the ideal treatment regimens in the laboratory as well as in clinical practice.

Paper Details

Date Published: 3 October 2014
PDF: 6 pages
J. Biomed. Opt. 19(10) 108002 doi: 10.1117/1.JBO.19.10.108002
Published in: Journal of Biomedical Optics Volume 19, Issue 10
Show Author Affiliations
Serena Incerti Parenti, Univ. degli Studi di Bologna (Italy)
Luigi Checchi, Univ. degli Studi di Bologna (Italy)
Milena Fini, Rizzoli Orthopaedic Institute (Italy)
Rizzoli Orthopaedic Institute (Italy)
Matilde Tschon, Rizzoli Orthopaedic Institute (Italy)

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