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Journal of Biomedical Optics • Open Access

Platform to investigate aqueous outflow system structure and pressure-dependent motion using high-resolution spectral domain optical coherence tomography
Author(s): Sepideh Hariri; Murray Johnstone; Yi Jiang; Steven Padilla; Zhehai Zhou; Roberto Reif; Ruikang K. Wang

Paper Abstract

The aqueous outflow system (AOS) is responsible for maintaining normal intraocular pressure (IOP) in the eye. Structures of the AOS have an active role in regulating IOP in healthy eyes and these structures become abnormal in the eyes with glaucoma. We describe a newly developed system platform to obtain high-resolution images of the AOS structures. By incorporating spectral domain optical coherence tomography (SD-OCT), the platform allows us to systematically control, image, and quantitate the responses of AOS tissue to pressure with a millisecond resolution of pulsed flow. We use SD-OCT to image radial limbal segments from the surface of the trabecular meshwork (TM) with a spatial resolution of ∼5  μm in ex vivo nonhuman primate eyes. We carefully insert a cannula into Schlemm’s canal (SC) to control both pressures and flow rates. The experimental results demonstrate the capability of the platform to visualize the unprecedented details of AOS tissue components comparable to that delivered by scanning electron microscopy, as well as to delineate the complex pressure-dependent relationships among the TM, structures within the SC, and collector channel ostia. The described technique provides a new means to characterize the anatomic and pressure-dependent relationships of SC structures, particularly the active motion of collagenous elements at collector channel ostia; such relationships have not previously been amenable to study. Experimental findings suggest that continuing improvements in the OCT imaging of the AOS may provide both insights into the glaucoma enigma and improvements in its management.

Paper Details

Date Published: 28 October 2014
PDF: 11 pages
J. Biomed. Opt. 19(10) 106013 doi: 10.1117/1.JBO.19.10.106013
Published in: Journal of Biomedical Optics Volume 19, Issue 10
Show Author Affiliations
Sepideh Hariri, Univ. of Washington (United States)
Murray Johnstone, Univ. of Washington (United States)
Yi Jiang, Univ. of Washington (United States)
Steven Padilla, Univ. of Washington (United States)
Zhehai Zhou, Univ. of Washington (United States)
Beijing Information Science and Technology Univ. (China)
Roberto Reif, Univ. of Washington (United States)
Ruikang K. Wang, Univ. of Washington (United States)


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