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Journal of Biomedical Optics • Open Access

Measurement of retinal blood flow in the rat by combining Doppler Fourier-domain optical coherence tomography with fundus imaging
Author(s): René M. Werkmeister; Martin Vietauer; Corinna Knopf; Clemens Fürnsinn; Rainer A. Leitgeb; Herbert Reitsamer; Martin Gröschl; Gerhard Garhöfer; Walthard Vilser; Leopold Schmetterer

Paper Abstract

A wide variety of ocular diseases are associated with abnormalities in ocular circulation. As such, there is considerable interest in techniques for quantifying retinal blood flow, among which Doppler optical coherence tomography (OCT) may be the most promising. We present an approach to measure retinal blood flow in the rat using a new optical system that combines the measurement of blood flow velocities via Doppler Fourier-domain optical coherence tomography and the measurement of vessel diameters using a fundus camera-based technique. Relying on fundus images for extraction of retinal vessel diameters instead of OCT images improves the reliability of the technique. The system was operated with an 841-nm superluminescent diode and a charge-coupled device camera that could be operated at a line rate of 20 kHz. We show that the system is capable of quantifying the response of 100% oxygen breathing on the retinal blood flow. In six rats, we observed a decrease in retinal vessel diameters of 13.2% and a decrease in retinal blood velocity of 42.6%, leading to a decrease in retinal blood flow of 56.7%. Furthermore, in four rats, the response of retinal blood flow during stimulation with diffuse flicker light was assessed. Retinal vessel diameter and blood velocity increased by 3.4% and 28.1%, respectively, leading to a relative increase in blood flow of 36.2%. The presented technique shows much promise to quantify early changes in retinal blood flow during provocation with various stimuli in rodent models of ocular diseases in rats.

Paper Details

Date Published: 16 October 2014
PDF: 9 pages
J. Biomed. Opt. 19(10) 106008 doi: 10.1117/1.JBO.19.10.106008
Published in: Journal of Biomedical Optics Volume 19, Issue 10
Show Author Affiliations
René M. Werkmeister, Medizinische Univ. Wien (Austria)
Martin Vietauer, Medizinische Univ. Wien (Austria)
Vienna Univ. of Technology (Austria)
Corinna Knopf, Medizinische Univ. Wien (Austria)
Clemens Fürnsinn, Medizinische Univ. Wien (Austria)
Rainer A. Leitgeb, Medizinische Univ. Wien (Austria)
Herbert Reitsamer, Paracelsus Univ. (Austria)
Martin Gröschl, Technische Univ. Wien (Austria)
Gerhard Garhöfer, Medizinische Univ. Wien (Austria)
Walthard Vilser, Imedos GmbH (Germany)
Leopold Schmetterer, Medizinische Univ. Wien (Austria)

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