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Journal of Biomedical Optics • Open Access

Mapping local orientation of aligned fibrous scatterers for cancerous tissues using backscattering Mueller matrix imaging
Author(s): Honghui He; Minghao Sun; Nan Zeng; E. Du; Shaoxiong Liu; Yihong Guo; Jian Wu; Yonghong He; Hui Ma

Paper Abstract

Polarization measurements are sensitive to the microstructure of tissues and can be used to detect pathological changes. Many tissues contain anisotropic fibrous structures. We obtain the local orientation of aligned fibrous scatterers using different groups of the backscattering Mueller matrix elements. Experiments on concentrically well-aligned silk fibers and unstained human papillary thyroid carcinoma tissues show that the m22, m33, m23, and m32 elements have better contrast but higher degeneracy for the extraction of orientation angles. The m12 and m13 elements show lower contrast, but allow us to determine the orientation angle for the fibrous scatterers along all directions. Moreover, Monte Carlo simulations based on the sphere-cylinder scattering model indicate that the oblique incidence of the illumination beam introduces some errors in the orientation angles obtained by both methods. Mapping the local orientation of anisotropic tissues may not only provide information on pathological changes, but can also give new leads to reduce the orientation dependence of polarization measurements.

Paper Details

Date Published: 15 October 2014
PDF: 8 pages
J. Biomed. Opt. 19(10) 106007 doi: 10.1117/1.JBO.19.10.106007
Published in: Journal of Biomedical Optics Volume 19, Issue 10
Show Author Affiliations
Honghui He, Graduate School at Shenzhen, Tsinghua Univ. (China)
Minghao Sun, Tsinghua Univ. (China)
Nan Zeng, Graduate School at Shenzhen, Tsinghua Univ. (China)
E. Du, Graduate School at Shenzhen, Tsinghua Univ. (China)
Shaoxiong Liu, Huazhong Univ. of Science and Technology (China)
Yihong Guo, Graduate School at Shenzhen, Tsinghua Univ. (China)
Jian Wu, Tsinghua Univ. (China)
Yonghong He, Graduate School at Shenzhen, Tsinghua Univ. (China)
Hui Ma, Graduate School at Shenzhen, Tsinghua Univ. (China)


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