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Journal of Biomedical Optics

Evaluation of transdermal delivery of nanoemulsions in <italic<ex vivo</italic< porcine skin using two-photon microscopy and confocal laser-scanning microscopy
Author(s): Sanghoon Choi; Jin Woong Kim; Yong Joong Lee; Thomas Delmas; Changhwan Kim; Soyeun Park; Ho Lee
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Paper Abstract

This study experimentally evaluates the self-targeting ability of asiaticoside-loaded nanoemulsions compared with nontargeted nanoemulsions in <italic<ex vivo</italic< experiments with porcine skin samples. Homebuilt two-photon and confocal laser-scanning microscopes were employed to noninvasively examine the transdermal delivery of two distinct nanoemulsions. Prior to the application of nanoemulsions, we noninvasively observed the morphology of porcine skin using two-photon microscopy. We have successfully visualized the distributions of the targeted and nontargeted nanoemulsions absorbed into the porcine skin samples. Asiaticoside-loaded nanoemulsions showed an improved <italic<ex vivo</italic< transdermal delivery through the stratum corneum compared with nonloaded nanoemulsions. As a secondary measure, nanoemulsions-applied samples were sliced in the depth direction with a surgical knife in order to obtain the complete depth-direction distribution profile of Nile red fluorescence. XZ images demonstrated that asiaticoside-loaded nanoemulsion penetrated deeper into the skin compared with nontargeted nanoemulsions. The basal layer boundary is clearly visible in the case of the asiaticoside-loaded skin sample. These results reaffirm the feasibility of using self-targeting ligands to improve permeation through the skin barrier for cosmetics and topical drug applications.

Paper Details

Date Published: 9 October 2014
PDF: 8 pages
J. Biomed. Opt. 19(10) 106006 doi: 10.1117/1.JBO.19.10.106006
Published in: Journal of Biomedical Optics Volume 19, Issue 10
Show Author Affiliations
Sanghoon Choi, Kyungpook National Univ. (Korea, Republic of)
Jin Woong Kim, Hanyang Univ. (Korea, Republic of)
Yong Joong Lee, Kyungpook National Univ. (Korea, Republic of)
Thomas Delmas, Capsum SAS (France)
Changhwan Kim, Kyungpook National Univ. (Korea, Republic of)
Soyeun Park, Keimyung Univ. (Korea, Republic of)
Ho Lee, Kyungpook National Univ. (Korea, Republic of)

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