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Journal of Biomedical Optics

Laser line scanning for fluorescence reflectance imaging: a phantom study and <italic<in vivo</italic< validation of the enhancement of contrast and resolution
Author(s): Frederic Fantoni; Lionel Hervé; Vincent Poher; Sylvain Gioux; Jérôme I. Mars; Jean-Marc Dinten
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Paper Abstract

Intraoperative fluorescence imaging in reflectance geometry is an attractive imaging modality to noninvasively monitor fluorescence-targeted tumors. In some situations, this kind of imaging suffers from poor resolution due to the diffusive nature of photons in tissue. The objective of the proposed technique is to tackle this limitation. It relies on the scanning of the medium with a laser line illumination and the acquisition of images at each position of excitation. The detection scheme proposed takes advantage of the stack of images acquired to enhance the resolution and the contrast of the final image. The experimental protocol is described to fully understand why we overpass the classical limits and validate the scheme on tissue-like phantoms and <italic<in vivo</italic< with a preliminary testing. The results are compared with those obtained with a classical wide-field illumination.

Paper Details

Date Published: 1 October 2014
PDF: 11 pages
J. Biomed. Opt. 19(10) 106003 doi: 10.1117/1.JBO.19.10.106003
Published in: Journal of Biomedical Optics Volume 19, Issue 10
Show Author Affiliations
Frederic Fantoni, Univ. Grenoble Alpes (France)
CEA (France)
Lionel Hervé, Univ. Grenoble Alpes (France)
CEA (France)
Vincent Poher, Univ. Grenoble Alpes (France)
CEA (France)
Sylvain Gioux, Harvard Medical School (United States)
Jérôme I. Mars, Gipsa-lab (France)
Jean-Marc Dinten, Univ. Grenoble Alpes (France)
CEA (France)

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