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Journal of Biomedical Optics

Real-time brightfield, darkfield, and phase contrast imaging in a light-emitting diode array microscope
Author(s): Ziji Liu; Lei Tian; Sijia Liu; Laura Waller
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Paper Abstract

We demonstrate a single-camera imaging system that can simultaneously acquire brightfield, darkfield, and phase contrast images in real time. Our method uses computational illumination via a programmable light-emitting diode (LED) array at the source plane, providing flexible patterning of illumination angles. Brightfield, darkfield, and differential phase contrast images are obtained by changing the LED patterns, without any moving parts. Previous work with LED array illumination was only valid for static samples because the hardware speed was not fast enough to meet real-time acquisition and processing requirements. Here, we time multiplex patterns for each of the three contrast modes in order to image dynamic biological processes in all three contrast modes simultaneously. We demonstrate multicontrast operation at the maximum frame rate of our camera (50 Hz with 2160×2560  pixels).

Paper Details

Date Published: 1 October 2014
PDF: 5 pages
J. Biomed. Opt. 19(10) 106002 doi: 10.1117/1.JBO.19.10.106002
Published in: Journal of Biomedical Optics Volume 19, Issue 10
Show Author Affiliations
Ziji Liu, Univ. of Electronic Science and Technology of China (China)
Univ. of California, Berkeley (United States)
Lei Tian, Univ. of California, Berkeley (United States)
Sijia Liu, Univ. of California, Berkeley (United States)
Laura Waller, Univ. of California, Berkeley (United States)

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